Cryopreservation

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Cryopreservation of embryos

The achievement of ovulation induction for assisted reproduction procedures and subsequent freezing of embryo practices are common in human reproduction centers. The “standard” protocol consists of approximately 10 days of ovarian stimulation with daily injections of gonadotropins, and applications should begin within the first three days of the menstrual cycle.

Sperm Cryopreservation

Also called freezing of sperm, cryopreservation of sperm is a very effective method that dates back to the 1940s. In patients with cancer, it is highly recommended that the sperm be collected before the start of cancer therapy (chemotherapy or radiation) because the integrity of the sample and the quality of sperm DNA can be compromised even after the first session. This process of cancer treatment – chemotherapy or radiation – can also lead to the decline and even ultimate extinction of sperm production. Given this situation, the use of IVF / ICSI (cytoplasmic sperm injection) enables success, even after defreezing, and with a limited amount of sperm.

This technique is also recommended for patients who wish to undergo vasectomy, in order to preserve fertility for the future. There are also more advanced options such as the extraction of sperm from the testis and testicular tissue freezing.

Oocyte Cryopreservation

In recent years, with the advent of vitrification technique, we observed a high success rate in the freezing-defreezing of oocytes, allowing women to have a new alternative for preserving their fertility.

With the technique of vitrification, the success rate of survival rose to 90%. The procedure follows exactly the same preparation used for freezing embryos until the extraction of oocytes, but instead of being fertilized, they are cryopreserved. The oocytes can be obtained by hormonal stimulation, and alternatively by in vitro maturation. The biggest advantage of oocyte freezing is that it is not dependent on partners.

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